Hi everyone, I am developing a tetra primer ARMS PCR. can you help me for designing primer?

03 March 2017 0 3K Report

I design several primer using "http://primer1.soton.ac.uk/primer1.html" website but I do not know if they work or not? would you explain me what criteria should i consider for best primer selection? for example what is the best length? acceptable dG for 3'hairpin?

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Science method

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