If you want a really cheap method just cut the band out of the gel, Freeze and thaw the gel 3 or 4 times and spin the band in agarose hard for 10 mins. The supernatant can be used for sequencing.

  Quite cheap is the exo sap method where you destroy the primers in the crude pcr mix with exonuclease1 and inactivate the NTPs with shrimp alkaline phosphatase. The kit is expensive but the reagents are cheap and the  reaction only takes about 20 mins and the product is ready to sequence

Note: I am aware of several Kit methods and ethanol purification.

Any other methods will help me.

Thanks

Greetings,

Re-use spin columns after several washing with warm sterile water then you can add 650 µl of Sephadex G-50 suspension. Let to set for 15 min. Spin for 2 min. Transfer to sterile eppendorf tube then add your DNA on the top center of the formed sephadex packed mesh. Spin for 2 min. Your DNA is clean now.

Regards,

If you want a really cheap method just cut the band out of the gel, Freeze and thaw the gel 3 or 4 times and spin the band in agarose hard for 10 mins. The supernatant can be used for sequencing.

  Quite cheap is the exo sap method where you destroy the primers in the crude pcr mix with exonuclease1 and inactivate the NTPs with shrimp alkaline phosphatase. The kit is expensive but the reagents are cheap and the  reaction only takes about 20 mins and the product is ready to sequence

Hi Sella gounder:-P

Just little addition to paul rutland ans.

Cut the band portion of the gel and put in the 2 ml clean tube. Freeze it at 0 C or below for 20 min. Then add 200 ul of PCR quality water and thaw the gel with hands and spin down the agarose at 10000 rpm for 10 mins. Collect the supernatant. Repeat the same thrice. Then the DNA collected in the supernatant can be precipitated out by adding 1.5 volumes of ice cold isopropanol. This could yield enough quantity of product for sequencing (40 - 50 % recovery is surely possible).