Dear Dural,
They can be found in secretory granules after autophagy, but never heard of a one poured outa cell.
Best
Mannan
Hi, Mannan thanks for your answer.
To be more detailed on my question
I am working on a gene predicted to be G-protein coupled receptor based on protein domain. During my experiment, I localized its site of expression using transcriptional reporter. When I constructed translation reporter the expression pattern was different.
The transcriptional reporter construct includes 2KB upstream sequence from translational start site tagged to GFP.
The translation promoter includes 2KB upstream sequence with the entire coding region tagged to GFP in C-terminal.
Why do I see this difference in expression pattern in vivo.
Is there any way to analyze the sequence using online bioinformatic tools to understand further. Your suggestions will be highly valued
Thanks
Durai
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