I am using the B16-F10 cell line as a model for melanoma in mice. after treatment, i see an increase in the ratio of M1/M2 macrophages in tumor tissue. i suspect that the treatment I use either reprograms M2 TAMs to M1 or increases M1 migration into tumors from other sites? I was thinking of using a drug (after the tumor reaches 100mm3 in size) to inhibit the recruitment of any new microphage into the tumor thus I can distinguish whether TAMs already in the tumor are reprogrammed to M1 or M1 are migrating from other sites to the tumor.
VB-201 is a small molecule inhibitor of monocyte migration. Article Inhibition of monocyte chemotaxis by VB-201, a small molecul...
Recombinant mouse Macrophage Migration Inhibitory Protein is available, too.
https://store.genprice.com/recombinant-proteins/mif-macrophage-migration-inhibitory-factor-mouse-recombinant-protein/
By decreasing extracellular acidity (e.g. bicarbonate) and decreasing intracellular alkalinity (e.g. amiloride) you will substantially decrease migration.
Another useful drug is dasatinib that inhibits Src phosphorylation and limits the cytoskeleton activity involved in migration.
- Badges
- Science topic
- Similar topics
- Biological Science
- Anatomy
- Tissue