How much Protease inhibitor do I need to add to my mouse serum sample?

I am using Protease Inhibitor Cocktails (VWR_M221-1ML). I reconstituted the inhibitor, but I am not sure how much of the protease inhibitor do I add to my sample. I am preparing the sample for a...

09 October 2019 9,615 3 View

Light-Emitting Diodes (LEDs)?

Combinations Light-Emitting Diodes (LEDs) and Light Intensity, their effect together on plants.

02 April 2019 2,745 1 View

After running with gel electrophoresis, How can distinguish between PCR products, wild type, heterozygous and homozygous?

After running with gel electrophoresis, How can distinguish between PCR products, wild type, heterozygous and homozygous?

31 December 2018 3,073 0 View

Do you think that the political situation can affect the interpretation of the Qoran?

The answers are important to be an idea and opinion helps to know the impact of political circumstances on the interpretation of the Qoran

10 November 2017 4,200 0 View

Are you have any papers, chapters and publications about Archaeological stone tools on Iraq, Middle East and surrounding ?

I need litureture survey about natural stones used by ancient human found in archaeological sites.

09 October 2017 9,597 5 View

Does any test (software program) to measure a mitochondrial membrane potential in the early embryonic stages?

30 January 2017 5,699 3 View

Hi, I want to ask about using acridin orange test for detect any damage in DNA in the sperms after thawing in domestic animals?

Hi, I want to ask about using acridin orange test for detect any damage in DNA in the sperms after thawing in domestic animals.

06 July 2016 7,062 4 View

What is the criteria I should follow to chose the journal to publish my study?

I have data about design metallic nanoparticles and their application in organic transformations

01 January 1970 4,357 4 View

Interpreted vs compiled programming language

Which is better in terms of speed interpreted or compiled programming language for the same application?

01 January 1970 5,349 1 View

What is the criteria I should follow to chose the journal to publish my study?

I have data about design metallic nanoparticles and their application in organic transformations

01 January 1970 6,989 5 View

Can't recognize exact phase and PDF card no of Silicon carbide in XRD?

We have deposited silicon carbide on carbon substrate using one PVD technique. Due to amorphous behavior of SiC at low deposition temperature, we performed heat treatment of those films at...

22 February 2021 2,072 3 View

Why at farmer level Cow/Buffalo are not done artificial insemination just after 2 month for yearly calving?

Physiologically in cow, oestrous heat again goes on the peak after 2 months but locally this is not observed and AI is after two months but done after at least 6 months.

17 February 2021 685 4 View

Possible reasons for high bioavailability after I.M and S.C. routes...?

What should be the possible reasons if we obtained more than 100% bioavailability of antibiotics (marbofloxacin) after I.M. and S.C. routes of administration in cattle species.

16 February 2021 971 4 View

For PC-3 cell line. How long before irradiation do I have to synchronize my cells to G0/G1?

I am trying to do a clonogenic assay for PC-3 after irradiation. I have to synchronize my cells by serum starvation but there's a optimal time I have to do that before irradiation. been looking in...

09 February 2021 9,480 1 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

How to do mono formylation of N,N-diethyl-3-methoxybenzamide?

I have attempted the formylation of the above mentioned compound using a very well reported protocol using secondary Butyl Lithium and TMEDA at - 78 degrees Celsius. I have done it quite a few...

08 February 2021 2,793 2 View

Grid connected inverter using virtual synchronous machine?

Hi everybody, currently, I am working on the grid-connected inverter. I want to make my grid-connected PV system a PLL-less system. I have studied some reachers are implementing a virtual...

07 February 2021 6,078 2 View

How to determinate phosphatidylcholine content in cow milk?

I would like to determinate the phosphatidylcholine content in cow milk. We only have a Shimadzu Prominence HPLC (High Performance Liquid Chromatography) with a fluorescent detector for the...

18 January 2021 8,370 6 View

How do i prove oocyte incompetence?

the is because the preliminary result of superovulation with (400-560 mcg) stimufol among unflushed cows did not yield any embryos 10 cows each were treated with graded doses of stimufol (400,...

13 January 2021 3,618 2 View

How to measure abundance in an LC-MS/MS experiment?

Is the chromatographic peak a valid measure of a compounds` (relative) abundance in LC-MS data? We have recorded three MS/MS spectra; their retention time falls into a specific chromatographic...

13 January 2021 6,685 6 View