How much Protease inhibitor do I need to add to my mouse serum sample?

I am using Protease Inhibitor Cocktails (VWR_M221-1ML). I reconstituted the inhibitor, but I am not sure how much of the protease inhibitor do I add to my sample. I am preparing the sample for a...

09 October 2019 9,615 3 View

Light-Emitting Diodes (LEDs)?

Combinations Light-Emitting Diodes (LEDs) and Light Intensity, their effect together on plants.

02 April 2019 2,745 1 View

After running with gel electrophoresis, How can distinguish between PCR products, wild type, heterozygous and homozygous?

After running with gel electrophoresis, How can distinguish between PCR products, wild type, heterozygous and homozygous?

31 December 2018 3,073 0 View

Can any researcher advise me how can I simulate dynamic loads on GEO-slope /Seep 2012?

Can any researcher advise me how can I simulate Wheel loads on GEO-slope /Seep 2012 as a simulation wheel load upon flexible pavements

10 November 2017 9,247 0 View

Dose the earthquake waves spread at the loose soil ?

Earthquakes

10 November 2017 8,358 0 View

What are the principles of learning and studying graphic design?

I want to know all the things that I should learn to become a graphic designer.

10 November 2017 9,012 0 View

Do you think that the political situation can affect the interpretation of the Qoran?

The answers are important to be an idea and opinion helps to know the impact of political circumstances on the interpretation of the Qoran

10 November 2017 4,200 0 View

Hi colleagues, Can any one give me information or articles about metabolomic in cow and ewe with estrus synchronization

Hi colleagues,Can any one give me information or articles about metabolomic in cow and ewe with estrus synchronization

04 May 2017 5,290 1 View

Does any test (software program) to measure a mitochondrial membrane potential in the early embryonic stages?

30 January 2017 5,699 3 View

Hi, I want to ask about using acridin orange test for detect any damage in DNA in the sperms after thawing in domestic animals?

Hi, I want to ask about using acridin orange test for detect any damage in DNA in the sperms after thawing in domestic animals.

06 July 2016 7,062 4 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

How can I separate Gertrud Pfister's profile and the profile of Gerald Gems?

we are co-authors of several publications. But I am the only and single author of most of my publications which are based on my own research. How can I separate the profiles?

21 February 2021 4,582 3 View

Why use both bead beating and enzymes (lysostaphin and lysozyme) for DNA extraction from sputum samples?

Hello, I have read in a few publications that to extract total DNA from sputum samples, both bead beating and enzymes are used to disrupt the cells. Bead beating is supposed to be efficient in...

18 February 2021 3,360 2 View

Change of First name?

In some publications you refer Deivid Chica (with Barranco) but the true first name is Maria Dolores Chica , in spanish Ma. Dolores Chica. Before I answer yes is an author Deivid Chica, but...

18 February 2021 5,004 3 View

Glucose 6 phosphate dehydrogenase deficiency & NSAIDs - is this combination harmless ?

G6PD deficiency is quite common in our anesthesia practice in the middle east. Intraop/postop analgesia in them is an issue here. Many websites/books give contrasting advise with former advocating...

10 February 2021 4,817 3 View

How should I compute a new variable with z-scores?

I would like to create a new variable using 4 different measures: hIndex (range: 1-47), # of publications (range: 0-111), and 2 other measures (range: 1-7). I changed all of these variables into...

09 February 2021 4,866 3 View

Do you know any reporting tool, guidelines for credibility assessment tool used for studies/publications with Artificial Intelligence?

I want to conduct a Systematic Review and assess the quality of methods used/proposed by researchers. I found out that scientists often utilize QUADAS for that...

05 February 2021 8,743 5 View

Do you need permission to use a middle range theory in your dissertation?

I am using a figure of a middle range nursing theory, do I need permission to use?

03 February 2021 2,210 9 View

How do Research Gate proceed to integrate in the reference data publications and citations the recently published journals ?

How do Research Gate proceed to integrate in the reference data for publications and citations the recently published journals - for instance for the last months of 2020 and for instance Intl J...

02 February 2021 9,336 7 View