After running with gel electrophoresis, How can distinguish between PCR products, wild type, heterozygous and homozygous?
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15 September 2023 6,211 3 View
Delve into the realm of critical stylistics and its profound impact on the interpretation of literary works. In this discussion, we'll explore how delving beyond the surface of language and...
10 August 2023 5,928 1 View
Hi All, I try to predict water demand based on previous water consumption. I use two models. The results of the fist model are: MAE=0.0109, CE=0.974 and MARE= 0.001105 The results of the second...
23 January 2020 7,662 4 View
I am using Protease Inhibitor Cocktails (VWR_M221-1ML). I reconstituted the inhibitor, but I am not sure how much of the protease inhibitor do I add to my sample. I am preparing the sample for a...
09 October 2019 9,762 3 View
Combinations Light-Emitting Diodes (LEDs) and Light Intensity, their effect together on plants.
02 April 2019 2,901 1 View
The answers are important to be an idea and opinion helps to know the impact of political circumstances on the interpretation of the Qoran
10 November 2017 4,295 0 View
I need litureture survey about natural stones used by ancient human found in archaeological sites.
09 October 2017 9,747 5 View
Hi colleagues,Can any one give me information or articles about metabolomic in cow and ewe with estrus synchronization
04 May 2017 5,452 1 View
I have daily data of clean water consumption, and I want to remove trend from the series by using HP filter method and Matlab programme. The programme suggests the following smoothing...
01 March 2017 7,697 3 View
After using JC-1 to stain mitochondria in the early embryonic stages, it should use specific software to analyze a polarized-mitochondria membrane potential but how can I analyze mitochondria in...
30 January 2017 5,804 3 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,953 0 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...
04 August 2024 6,033 0 View