How much Protease inhibitor do I need to add to my mouse serum sample?

I am using Protease Inhibitor Cocktails (VWR_M221-1ML). I reconstituted the inhibitor, but I am not sure how much of the protease inhibitor do I add to my sample. I am preparing the sample for a...

09 October 2019 9,615 3 View

Light-Emitting Diodes (LEDs)?

Combinations Light-Emitting Diodes (LEDs) and Light Intensity, their effect together on plants.

02 April 2019 2,745 1 View

Do you think that the political situation can affect the interpretation of the Qoran?

The answers are important to be an idea and opinion helps to know the impact of political circumstances on the interpretation of the Qoran

10 November 2017 4,200 0 View

Are you have any papers, chapters and publications about Archaeological stone tools on Iraq, Middle East and surrounding ?

I need litureture survey about natural stones used by ancient human found in archaeological sites.

09 October 2017 9,597 5 View

Hi colleagues, Can any one give me information or articles about metabolomic in cow and ewe with estrus synchronization

Hi colleagues,Can any one give me information or articles about metabolomic in cow and ewe with estrus synchronization

04 May 2017 5,290 1 View

Does any test (software program) to measure a mitochondrial membrane potential in the early embryonic stages?

30 January 2017 5,699 3 View

Hi, I want to ask about using acridin orange test for detect any damage in DNA in the sperms after thawing in domestic animals?

Hi, I want to ask about using acridin orange test for detect any damage in DNA in the sperms after thawing in domestic animals.

06 July 2016 7,062 4 View

What is the criteria I should follow to chose the journal to publish my study?

I have data about design metallic nanoparticles and their application in organic transformations

01 January 1970 4,357 4 View

Interpreted vs compiled programming language

Which is better in terms of speed interpreted or compiled programming language for the same application?

01 January 1970 5,349 1 View

What is the criteria I should follow to chose the journal to publish my study?

I have data about design metallic nanoparticles and their application in organic transformations

01 January 1970 6,989 5 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View