I am looking to use this method for my PhD research. Have attached my proposal, would love some support, currently working my way through ethics.
Dear all, I would like to kindly ask for any recommendations on how to extract pure RNA from honeybee brains. After snap-freezing whole bees in liquid nitrogen, I have been smashing the whole...
15 May 2024 7,305 4 View
Hi, I have recently started ALI culture of Calu3 cells. Before the airlift my cells grew to a confluent monolayer with a nice TEER value, but after airlift and change to ALI maintenance media from...
21 November 2023 6,664 2 View
Hi, We are looking to culture our mES cell lines in 2i media. We currently use either classic ES cell culturing conditions (FBS, LIF, GSK inhibitor and MEFs) or serum free KnockOut cell...
17 November 2023 340 1 View
Has anyone encountered the error "Reader Error: 5402 Read head Z failed positional verify" using the logphase 600? And have any ideas on fixes for it? We're using the plate reader for monitoring...
01 May 2023 6,322 0 View
May I share a link to the article being requested? It is free for download (so not copyright restricted) on the website of the Brookings Institution. Also, I see that 8 people have requested...
07 March 2023 9,095 0 View
I am fairly new to syntax writing/coding and need to merge many excel files (with the same set of variables) into one SPSS dataset but it must be done with syntax. So far, all of the loops for...
31 March 2022 6,635 3 View
Hi everyone, I’m facing some issues regarding the implementation of an external material routine in COMSOL. The aim is to provide a general stress-deformation routine for an elastic-ideal plastic...
19 February 2022 4,316 0 View
I have a gemini quaternary ammonium salt that also contains two secondary amide groups, which I need to reduce to amines. I tried using LiAlH4 in 6 eq. in anhydrous THF at 66 °C, but that...
25 January 2022 5,879 4 View
Hi, I'm having problems finding CREB and pCREB antibodies that tag the same proteins in my blots. (see attached) We are testing between 5 and 15ug protein loads from mouse hippocampal cell...
22 January 2022 232 1 View
I am looking for either a commercial source or a lab that has propagated RSV in another cell line other than HeLa. Thanks in advance!
14 December 2021 5,310 1 View
I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...
12 August 2024 862 0 View
“Here is a thought experiment. Let's place Rodolpho Llinas's jarred-brain on top of a body (Fig. 1). I bet Llinas would argue that his jarred-brain retains its own consciousness, and the android...
11 August 2024 2,483 1 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...
08 August 2024 8,964 4 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
So I am organizing an AI seminar. What are possible AI projects in the AI for good spirit? something the students can do and have an impact?
08 August 2024 9,437 4 View
The rate of glucose consumption by the neocortex is reduced by over 80% during anesthesia (Sibson et al. 1998), which disables the synapses (Richards 2002) that are inundated by glial tissue (Engl...
08 August 2024 3,118 0 View
How to design human-centered classroom in the age of A.I.?
08 August 2024 347 5 View
I am trying to analyse data from a survey examining what variables affect teachers perceived barriers to incorporating technology into their classroom. I have 5 predictor variables however my DV...
06 August 2024 1,752 3 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View