I am planning to develop a Human T cell line having the potential to kill OVA expressing tumor lines.

Mostly we have to take out Tcells from OT1 mice which is expensive in overall cost.

If I can, kindly suggest the best plasmid for the same.

Or can I plan immortalization of OT1 mice T cells?

So that I will not need to sack the mice again and again.

Which of the task will be more feasible..

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