I'm looking forward to improve some stainings in mice CNS (brain and bone marrow). I'm intersted in motor neurons and their extensions. My purpose is to count and compare them in diferent samples.
Thank you!
See, for example:
Sci Rep. 2015 Nov 20;5:16953. doi: 10.1038/srep16953.
Adipose-derived Stem Cell Conditioned Media Extends Survival time of a mousemodel of Amyotrophic Lateral Sclerosis.
Fontanilla CV1, Gu H1, Liu Q2, Zhu TZ1, Zhou C2, Johnstone BH3, March KL3,4, Pascuzzi RM1, Farlow MR1, Du Y1.
Hi there
toluidine blue is also similar to H and E staining.
Good Luck
Cresyl violet staining is applicable for nissle body staining of neuron.
Thank you very much everybody!
I'll show you my results.
;)
I am looking for ways to produce powder from an aluminum bar. I can't find an available grinder/mill, so I am looking for an alternative. I saw a method wherein a steel plate and a steel bar were...
07 June 2016 3,949 15 View
Hello! I am trying to recover pure aluminum powder from metal shavings contaminated with oil and coolant. Can I simply evaporate the oil and coolant to obtain the aluminum shavings then afterwards...
12 May 2016 7,766 14 View
I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...
03 March 2021 4,116 4 View
Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...
03 March 2021 961 3 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.
02 March 2021 1,516 4 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...
28 February 2021 7,800 6 View
Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...
27 February 2021 5,028 2 View
Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...
27 February 2021 3,904 2 View
I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...
27 February 2021 4,218 7 View
Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...
26 February 2021 9,119 3 View