Im trying to measure lipid peroxidation using fluorescent probe BODIPY FL C16 (Molecular Probes) using flow cytometry in plasmodium falciparum infected erythrocytes. But Im having troubles analyzing my results. I found that both green and red fluorescence decrease after my induction treatments comparing to control, but my intensity ratios increase. I found in literature ambiguous ways to analyze same data. Some just analyze an increment in green/red ratio, others look for an increase in green fluorescence and others looks for a decrease in green fluorescence. Im a little confused. If anyone has some experience with this dye and any suggestions they will be well received.

Thanks in advance!