I need to convert Avicel into cellobiose (no glucose), for which I need well compatible and efficient pair of endo and exoglucanase (no beta-glucosidase in the mixture). I would welcome ideally something sold by Sigma-Aldrich.
Think you should look for products from Megazyme. They have many carbnohydrate actyive enzymes and a good characterization.
cordially
Thank you Catherine, I'll check this option. Anybody else with even more specific suggestion?
Hi Pavel, I've been using the commercially available cellulase (endo-1,4-β-D-glucanase) (Bacillus amyloliquefaciens) (Megazme) to enzymatically produce gluco-oligosaccharides (mainly cellobiose, cellotriose, cellotetraose) from a cellulose-enriched pretreated pulp. This cellulase has a high enzyme activty, so doesn't require large enzyme dosages/volumes. Please note that you do end up with small amounts of glucose as a side product during enzymatic hydrolysis as the endo-glucanase mix cleaves the cellulose chain into smaller cello-oligomeric fragments. For instance, cellotriose can be enzymatically cleaved to cellobiose and glucose. I haven't measured β-glucosidase or cellobiohydrolase activity for this endo-glucanase. Also, if you want to avoid enzymatically generating glucose, I suggest you evaluate short enzymatic hydrolysis times as well as low enzyme loadings.
Link of the endo-glucanase below:
https://www.megazyme.com/cellulase-endo-1-4-beta-d-glucanase-bacillus-amyloliquefaciens
Hope this is of help.
Cheers,
Rakesh.
Also forgot to mention that the above endo-glucanse from Megazyme will only produce gluco-oligosaccharides selectively from amorphous cellulose or cellulose II, and not crystalline cellulose. So this endo-glucanase is not suitable for untreated microcrystalline Avicel. The paper/mini-review in the link below highlights strategies to generate gluco-oligosaccharides:
https://onlinelibrary.wiley.com/doi/full/10.1002/pi.5398
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