Hi, can any one suggest me the suitable animal model for induction of diabetic complications.
Asimple way is to induce diabetes in rats or mice, using chemicals such as alloxan or streptozocin; and then study the complications that occur in those animals as the disorder runs its course.
Thanks alot for your concern, but the problem i have faced during previous studies on diabetic complication is mortality and reversibility of hyperglycemic condition in rats therefore I want to know how to overcome these issue to study the diabetic complications
Anwar,
Your observations seems to be a matter of your experimental design.
If you use excessive amount of STZ, animals are very likely to die.
If you use correct amount of STZ (correctly) to destroy all (or nearly all) beta cells, they will never revert to normoglycemia. But then you need to provide some insulin to sustain their life in the long-run.
Dear Mirza anwar
If you fixing the dose of STZ 60mg/kg with Nicotinamide 120mg/kg. Then you can avoid the mortality. I hope the below contents are useful to you. Thank you.
There are some
disadvantages to its use in chronic experiments,
especially- spontaneous recovery from high blood
glucose levels by the development of functioning
insulinoma (Steiner et al., 1970; Yamagami et al.,
1985; Iwase et al., 1991) and high incidence of
kidney and liver tumours. These problems are due
strongly to oncogenic action of STZ (Kazumi et al.,
1978). Non insulin dependent diabetes mellitus
(NIDDM) was induced by a single intraperitoneal
injection of streptozotocin (60mg/kg) and
nicotinamide (120mg/kg) to rats (Pellegrino et al.,
1998). Paik et al. (2008) reported that, injection of a
single high dose of streptozotocin (200 mg/kg body
weight) induced rapid and permanent
hyperglycaemia in mice. In contrast, the injection of
the same total dose divided into multiple
“subdiabetogenic” doses (40 mg/kg per day for five
consecutive days) caused the development of
delayed but progressive hyperglycemia only in the
thymus competent mice. Female mice were less
susceptible to streptozotocin at both doses.
These articles can guide you on the induction of diabetes in animals
The rats of strain Wistar are suitable for the induction of experimental diabetes.
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