Some body transfect the T. gondii tachyzoites using complicate method/s I am looking for a simple and more applicable method for transfection of siRNA to this protozoa. Is there any experience?
What have you tried? You would have to get extracellular parasites, typically by passing the host-infected cells through a 21 guage needle, then centrifuge, resuspend in the right buffer, and then choose your method for introducing the siRNA. I only have experience with electroporation of DNA, not siRNA, but the method looks similar. See the publication below: