I was planning to do the universal protein extraction protocol by Wu et al. (2014) combining the TCA/acetone and phenol extraction techniques, but can you suggest a much easier way to extract protein from the adult leaves of a metal hyperaccumulating plant.
Houda Kawas
Dear Roneil Alonday
Pls. find the attached files
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3724048/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3778397/
page 454 in: Monika Waksmundzka-Hajnos, Joseph Sherma. 2010. High Performance Liquid Chromatography in Phytochemical Analysis. CRC Press,Pp.996. ISBN1420092618, 9781420092615
http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v6n3-6/617
Hoping this will be helpful
Regards
Prof. Houda Kawas
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