I have purified protein from various parts of the same plant. They have different concentrations. Do i need to "normalize" the concentration of all the purified proteins before digestion? or just proceed to the digestion with different concentration of proteins?
If you purpose of study is to analyse your proteins composition from different parts and to compare your results >>> you should use the same concentration to facilitate the comparaison.
If you are working on proteases activity, then the protocol of enzyme activity measurement will guide you.
In general, it's preferable to use the same concentration.
Good luck Roneil
If you want to study the particular protein quantity out of total protein and compare with the proteins of other plant parts definitely you should normalize
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