Roneil Alonday

4 Questions 2 Answers 0 Followers

Questions related from Roneil Alonday

Do I have to adjust the concentration of my purified proteins before enzymatic digestion?

I have purified protein from various parts of the same plant. They have different concentrations. Do i need to "normalize" the concentration of all the purified proteins before digestion? or just...

11 November 2017 5,183 3 View

Can I re-add fresh enzyme to the solution where I have previously digested for two hours but inactivated via boiling?

I am doing enzyme digestion for my bioactive peptide research. I was digesting for 2 hours when I accidentally inactivated the enzyme in my digestion mix. Can I continue the digestion by re-adding...

23 October 2017 9,898 3 View

Hi, aside from phenol extraction, what other protein isolation protocols can you suggest when dealing with adult recalcitrant leaves?

I was planning to do the universal protein extraction protocol by Wu et al. (2014) combining the TCA/acetone and phenol extraction techniques, but can you suggest a much easier way to extract...

15 October 2016 7,031 3 View

Hi, does anybody know a protocol when collecting plant samples from the field for proteomic studies?

I would like to collect samples (leaves, stem and roots) from the field for my proteomics study,

08 March 2016 5,257 3 View