Are there papers on?

I'm looking for a papers concerning either Christianity or neopagan religions and social media. What role does social media play in the spread of these religious movements? Any suggestions on the...

15 February 2021 655 4 View

Which commercial DNAse do you recommend me?

Which is the best DNAse (or DNAse kit) you have worked with? I am looking for a good DNAse that can remove DNA from an RNA sample in one pass. Material would be used for future RT-qPCR analysis....

18 November 2020 8,769 3 View

How to extract big peripheral membrane proteins?

I have been trying for a long time to see by Western Blot a 450 kDa-peripheral membrane protein from the Golgi apparatus with no success. I know that the electrophoresis and the membrane transfer...

28 September 2020 9,664 3 View

Maiden vs married name?

When I claimed authorship of papers written under my maiden name, they have now appeared under my married name, and I am worried this will cause confusion. How do I fix this issue?

12 August 2020 6,936 5 View

Rat DRG survival issue - how can I debug the protocol?

Hello everyone, I have been trying to establish primary rat DRG neuron culture for a couple of months already. I am using the protocol I found somewhere during the Google search, apparently...

14 June 2020 343 1 View

How to clean analytical size exclusion column?

Hello, We are using Superdex75 increase 3.2/30 GL size exclusion column in our lab and recently column pressure is to high to work on recommended flow rate 0.1 mL/min. I tried to clean the column...

19 May 2020 8,549 4 View

Unexpected problems running Photoquad software: How to fix the issue when it doesn't run anymore?

Hi, I've been using Photoquad software to analyze photoquadrats but suddenly the program doesn't run anymore and I am not able to solve the problem. I uninstalled both the MATLAB Compiler...

21 April 2020 4,707 1 View

Did any of you use polyethylene glycol to break down antibodies in Western Blot?

Hi, I've got a problem with my Western Blot. I have a low weight protein to detect but I suspect that my primary and secondary antibodies are clumping into a large ball at a higher molar mass than...

09 March 2020 2,856 3 View

Can anyone tell me the results of European Xenome research?

I know that the European Union has funded XENOME, an integrated project within the Sixth European Framework Programme (Life Sciences, Genomics and Biotechnology for Health), lasting 5 years...

18 November 2019 6,532 2 View

Long-to-wide with missing data...any help?

Hi! I am handling with multiple measurements at different time points and I want to transpose the dataset so that each measurement will be a variable and each subject would correspond to a single...

23 July 2019 8,387 3 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

03 March 2021 5,360 2 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

Physical-chemical formation of stalactites/stalagmites. what promotes the formation/growth of these structures?

I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...

22 February 2021 1,400 4 View