Hi all, is it possible to use XBridge BEH Amide Column to separate acetoin and 2,3-butanediol via HPLC?

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Can I change concentration and brand of antibodies mid-experiment?

I am doing immunohistochemistry of the c-Fos protein and I have a budget problem. For context, the experiment has been done over 3 years prior to my arrival and I have 40 brains done and 100 left....

29 July 2024 5,961 2 View

Paired t-test or unpaired t-test for my quantitative data with SPSS?

I am conducting a qualitative-driven approach to mixed-method research. The role of my quantitative data is to corroborate the findings of the qualitative data. Qualitative data has been collected...

24 July 2024 9,799 3 View

Have you combined pragmatism and interpretivism before?

Has anyone combined pragmatism and interpretivism in their research? If so, could you please recommend some reading to help with this? I am currently writing the methodological chapter for my PhD...

21 May 2024 2,639 2 View

¿Está la escuela española preparada para que se lleve a cabo la inclusión real de todo el alumnado?

En el informe TALIS 2018: Estudio Internacional de la enseñanza y aprendizaje dirigido a la mejora de la formación y el ejercicio docente, se han incluido aspectos relacionados con la innovación y...

11 May 2024 5,550 0 View

What is the best medium for Caco-2 cell line?

Dear Researchers, I would like to start working with Caco-2. I have seen in articles that different media (e.g. MEM or DMEM with various suplementation) are used to culture Caco-2. Which is the...

29 April 2024 4,949 2 View

Can anybody suggest a positive control for detecting phospho specific AKT(Ser473) in Flow Cytometry?

I want to check the phospho-AKT level in T cells by Flow . So, i am looking for a good positive control. Thank you

17 April 2024 4,449 0 View

A good book about disturbance ecology?

Hi I am trying to find a good, classical book or review about disturbance ecology. Any advice would be very helpful. Thanks in advance!

16 April 2024 4,642 1 View

The black pellets after protein precipitation?

Good morning, I used TCA/aceton protocol to precipitate proteins. I incubated vesicles from yeast with TCA. After centrifugation 14000xg, 10min at 4°C I saw black pellets in each samples... What...

21 March 2024 1,670 4 View

¿Cómo puedo informar correctamente aceerca de un posible caso de trata?

Todo el mundo alega que si ocurriese una injusticia lo correcto sería contarlo pero, cómo se debería dar voz de forma adecuada en este caso, sin poner en riesgo a las víctimas?

10 March 2024 5,202 1 View

¿Es lo mismo trata de personas que tráfico de personas?

Normalmente se suelen utilizar esos dos términos coloquialmente a la hora de hablar sobre el tema, y me gustaría saber las diferencias para llevar el tema con propiedad y que más personas sean...

10 March 2024 2,567 1 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Hi, please what is the best tool on Aspen plus that can help me get the best inlet temperature and pressure for cryogenic distillation?

I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...

06 August 2024 4,611 0 View

How to perform EEG source analysis on each trial of data separately?

Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...

30 July 2024 2,744 1 View

How to remove/ replace trifluoroacetate countertop after deprotection?

After deprotection of the tert-butyl ester to generate the carboxylic acid in chloroform with TFA, my compound‘s pyridine moiety forms a TFacetate ammonium salt (small molecule drug, not a...

29 July 2024 9,753 3 View

A positively charged and 10x his-tagged protein that doesn't bind to any chromatographic resin?

Hello everyone, I am currently working on a protein that is 10x his-tagged and positively charged (predicted pI=10.16). But when I tried to use Ni-NTA column to purify the protein, it's not...

24 July 2024 7,293 4 View

Why is trifluoroacetic acid (TFA) used in c-18 column?

I would like to know the role of the TFA (trifluoroacetic acid) used on reverse phase chromatography c-18 column. It's is often used with a concentration of 0.1% on the mobile phase.

23 July 2024 8,075 3 View

Standard population: What standard population to implement?

Dear all, I have a question about standard population? Is Segi population outdated? I have a time series from 2000 onward. Regarding who 2000-2025 population from this link...

13 July 2024 4,380 2 View