The enzyme will be fine and still active and all of the reagents will be stable at the annealing temperature.I would expect a slightly higher level of non specific amplifications but if the pcr always amplifies clean then if you reset to the proper parameters I would expect the pcr to work

I did that once and it turned out as the biggest mistake with nonsense amplification with the cost of my day, sample, chemicals. Please run that again to get specific results.

Should be OK, because the enzyme are stable. Agreed with Paul and Laurence, thanks.