How can I know the stationary phase of growth of several bacteria?

How can I know the stationary phase of growth of several bacteria? We are aiming to collect exoproteome of several bacteria. We know that the ideal time to collect exoproteome is during the...

31 January 2021 4,528 10 View

How to interpret the following contour plot?

Hi. I am new to Countour plots. I need help with the understanding of the following contour plot. If someone can explain, please.

10 November 2020 3,710 1 View

Qiagen DNeasy vs QIAquick spin column?

Dear Fellows I wanted to confirm if I can use spin columns of QIAquick PCR purification kits for DNA extraction. We finished our DNeasy columns but still have reagents. And we have extra QIAquick...

03 November 2020 6,960 3 View

Which method is suitable for transgenic plant development: In-planta transformation or tissue culture-based transformation?

Which method is suitable for transgenic plant development: In-planta transformation or tissue culture-based transformation?

13 September 2020 7,717 11 View

Transmission electron microscope (TEM) service available in India?

I want my biological sample to be analysed using TEM as we don't have that instrument. So can anyone suggest any institution or lab that provides cheap TEM experimental service in India.

10 August 2020 3,807 3 View

How to find Slow and Fast light?

I am facing a problem to calculate the Slow and Fast light using Matlab. Can some share a simple code for Slow and Fast light. Thanks Xie Xie Danke.

13 June 2020 3,955 2 View

Which modelling technique to predict corona virus trends in future?

Hi, I am working on time-series data for the last 2 years. However, I have never used forecasting models. I want to enhance my statistical skills. As corona is all around us, I was thinking to...

29 March 2020 7,452 6 View

Can we assume linearity of association from this graph?

Linearity is pretty simple and obvious to guess. However, in some cases, the scenario gets totally different. IF you see the following attached graph, would you consider it linear association or...

15 January 2020 7,901 3 View

What are the skills that a PhD researcher should learn to secure a job after PhD in research field?

Hi, As the research field is really competitive, so what are the skills recommended for a Ph.D. student to learn while doing a Ph.D.? For example, I am doing a Ph.D. in environmental health...

13 January 2020 6,598 26 View

Does statistical significance matters in interpretation of results for time-series analysis?

In time-series ecological study, do we take positive results (but statistically non-significant) serious enough to be generalized in the real population?

06 January 2020 4,236 9 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How to get better phase shift in C-V curves for ferroelectric devices?

I tried to sweep my ferroelectric devices from -2V to 2V and then from 2V to -2V and The two hysteresis peaks are off by several microFarads. Should i increase bias voltage? I am doing these...

16 February 2021 8,514 2 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

How to estimate/know plant virus concentration to make a standard curve in ELISA after serial dilution?

Hello, I am trying to know tomato virus titer/concentration in samples and I will perform serial dilution of the positive control. I wanted to ask How to estimate/know plant virus concentration to...

13 February 2021 6,534 4 View

What are the possible effects of COVID -19 Vaccine/s on the persons already recovered from the disease?

Persons recovered from COVID -19 are having antibodies against the virus inside their bodies. They are having T cells already sensitized to the antigens of the virus. If they are vaccinated (may...

12 February 2021 4,106 22 View