Is it possible to quantify calcium carbonate from XRD data?

I have not done the analysis but planning to. I want to estimate CaCO3 in algal biomass.

03 April 2018 6,018 5 View

Anybody experienced in using MPN for enumeration of microalgae?

I would like to know how the positive tubes are scored in MPN for microalgae. Fluorometry or chlorophyll a or any other plating method ?

31 December 2016 5,603 4 View

Inverted fluorescent microscope on lease. Is this service available in India?

08 September 2016 6,891 1 View

Anybody know of a scientific supplier who can supply a basic model of 22 litre autoclave(pic attached)?

07 August 2016 1,898 3 View

Is it possible to increase concentration of a v/v solution without increasing volume? If so, how can this be achieved?

I am working on culturing algae with effluent. I want to scale up the effluent concentration from 10% to 100% in sea water keeping the total volume constant. Will it be possible?

03 April 2014 7,495 1 View

SEM and TEM facilities in and around Tamil Nadu, India?

Can you guys list the institutions which provide SEM and TEM service for biological specimens?

07 August 2013 4,790 15 View

Inverse peaks in HPLC

Hi I was separating carbohydrates by RP-HPLC. I used to get the solvent peak before the analytes and it was normal. All of a sudden, the solvent peak got inversed. I don't know why? Can anyone...

11 December 2011 469 11 View

Hydroxyapatite chromatography

In hydroxyapatite chromatography of proteins, is it necessary to bake the slurry overnight in an oven? Has anybody performed hydroxyapatite chromatography of proteins? If so please guide me....I...

10 November 2011 8,746 0 View

Sugar preparation for HPLC

Do anyone know a method for sugar extraction from cyanobacteria for HPLC analysis?

08 September 2011 2,944 11 View

Sugar analysis

Anybody worked/working in cyanobacterial/microalgal sugar analysis by HPLC

08 September 2011 1,758 0 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to solve error "Cannot find position restraint file restraint.gro (option -r)." on GROMACS?

Hi, I am about to do energy minimization but the above error occurred, I checked my .mdp file and .top file but could not find the problem. TOP file ; Include forcefield parameters #include...

01 February 2021 780 3 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

Any stable dye to color acrylamide hydrogel beads?

Hi! We are generating hydrogel beads(50-60um) using microfluidics as single-cell beads. To simply all the experiments (especially the centrifuge), I am looking for a dye. The dye should be stable...

14 January 2021 4,383 8 View

How to phosphorylate specific residues on pymol?

Hi everyone, I am using the pyTMs plug in on Pymol to phosphorylate a particular threonine in my protein. I am having difficulty in selecting only one or two residues - it seems I can only...

05 January 2021 230 3 View