I ask you to help me, as I need to identify the genetic sequences that characterize the groups in the roary pangenome tool. It generates a file with present and absent genes and I need to know the unique genes of the strain I am working with. In fact, I have a file that filters these corresponding sequences and genes or groups of genes, but I wanted to know how I find the sequence information itself, so I know which sequences are classified within each group. Everything mentioned above is to create gene ontology, because I compared the genes of my strain with others and I want to see what makes them different. Then I'll do this OG. Can anyone tell me about roary, or an alternative option?
Thanks