I have isolated metagenomic DNA from1 gram of soil and dissolved in 200 mcl water. I took 100 mcl from it and added 2900 mcl water. Then I have transfered in cuvette and took od it gave me 0.431. It translates to 21.55 mcg/ml DNA, Do i need to multiply by 3 to calculate the amount of DNA in 100 mcl, since the assay volume is 3 ml ? and then multiply by 2 to calculate per gram of soil ? This may be a silly question. help me. Thanks
Yogesh Chaudhari
Hii..Dinesh..You are going in right direction..DNA conc. (ug/ml)= OD260 x dilution factor x 50 or else you can try
Concentration (µg/ml) = (A260 reading – A320 reading) × dilution factor × 50µg/ml
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