Dinesh S L

3 Questions 4 Answers 0 Followers

Questions related from Dinesh S L

How to carry out taxonomic classification of nifH functional gene ?

1. There are several database containing nifH sequences like fungene (19500 seq), arb database (32,954 seq), zehr (72000 seq) and farnelid (5,00,000 seq). what is the difference between these...

02 February 2020 689 3 View

Help me in DNA quantitaion per gram of soil ?

I have isolated metagenomic DNA from1 gram of soil and dissolved in 200 mcl water. I took 100 mcl from it and added 2900 mcl water. Then I have transfered in cuvette and took od it gave me 0.431....

02 February 2015 1,175 1 View

How can I normalize the sequence data of samples having different number of sequences ?

I know that not doing this step could result in obvious bias while calculating richness and ecological indices. I have sequenced using 454 and did all the analysis using pyroRDP. I would like to...

12 December 2014 1,849 8 View