I'm looking for a way to remove carotenoids from a solution containing chlorophylls and carotenoids, except chromatography method.
thank you.
There are methods in:
Rice, Eugene W, et al. Standard Methods for the Examination of Water and Wastewater. 22nd ed., American Public Health Association, 2012.
Article Carotenoids: Isolation and analysis
Article Methods of Analysis (Extraction, Separation, Identification ...
If instead you want to remove the chlorophyll:
Extract in 80% acetone and add 50% volume of 1 M KOH solution to induce alkaline hydrolysis, then shake or vortex the solution for several minutes at room temperature.
This procedure releases the phytol chain from chlorophyll resulting in chlorophyllides, which is far more polar than chlorophyll, so you can use a less polar solvent (e.g. petroleum ether or hexane) for solvent extraction of carotinoides by shaking, vortexing or centrifugating.
Any way the upper phase will contain just carotinoids and the lower chlorophyllides.
Nicolò Maria Villa , Thank U for your good answer, but in fact my goal is to study
extracted chlorophylls and presence of carotenoid makes overlap.
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