Hello, What volume of urea (4M and 8M) is best for denaturation of peptide-MHCI and/or Peptide-MHCII complexes?

23 July 2022 0 1K Report

I plan to use the Neoscreen assay as described here (Article Identification and validation of 174 COVID-19 vaccine candid...

); however this and many other studies which used same/similar methods did not mention the volume for Urea.

Thank you

Shah

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