Molecular Biologists, Immunology
This is the first time I need quantitative results for ELISA. What kind of methods are there? Which one is the best? Thank you.
05 June 2016 4,166 3 View
I do transformations of erythromycin resistant plasmids into S.mutans. I am short in time. Do you know what is the working concentration of S.mutans UA159 to erythromycin? I tried with 10 ug/ml,...
08 September 2015 9,231 1 View
I have to transform pet21 vector into S.mutans U159 strain. Did it work in your case?
07 August 2015 9,063 2 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Could someone please confirm if those vacuolated structures are goblet cells? And whether the sections exhibit signs of inflammation. Context: I am conducting histological analysis of the...
15 June 2024 3,208 4 View
define cd
06 June 2024 9,533 5 View
I'd like to receive notifications of call for papers for any journal pertaining to my expertise (immunology, rheumatology, immuno-mediated liver involvement) from researchgate. Thanks in advance...
01 June 2024 1,352 0 View
If I want to put mouse light chain(Sac1 and XbaI) and heavy chain (XhoI and SpeI) in pComb3XSS (phagemid) to form Fab, should I add stop codon at the light chain?
20 May 2024 806 0 View
Hi all, I'm trying to validate an ELISA against anti-drug antibodies in which I use a immunized rabbit serum as positive control. Rabbit was a NZR, immunization protocol was "standard" but I...
13 May 2024 2,828 0 View
For my project, I am attempting to purify an intrinsically disordered domain cloned in a pET28b vector. The strain used for overexpression is Rosetta II(DE3). Firstly, I encountered an unexpected...
23 April 2024 9,939 4 View
Hii all.. I have few sequences of Fusarium spp partial TEF region. I want to submit them to genebank to get accession numbers. Can someone please help how to submit sequences of coding regions? It...
23 April 2024 2,468 6 View
