You should be able to simply highlight the samples you want to include as reference (by checking their boxes) and click on "distance tree of results".

Dear Drifa

It's very simple. You first need to perform a BLAST analysis of your sequence, and then select the species/strains with which you want to compare. In the next step, select the "distance tree of results" as mentioned by Alex.

Good luck

first of all do the sequencing of your product then do BLAST on NCBI,

u will get information about that strain.