Best way will be you need to give a diagrammatic sketch (even drawing by hand) the orientation of brain where the cut is made, that is Key. You should always keep in mind the proper orientation of brain tissue otherwise your publication will not be accepted. If you can give that orientation, someone can easily answer your question, good luck
mostly nerve fibers. brain should bee examined after perfusion fixation for proper examination and good results
Dear Alaa Skeyni ,
rat, mouse...(rodents...): Not knowing about the specific area your section comes from and not knowing the "orientation of section plane":
my guess:... perhaps part (tangential section plane, especially when sectioning coronal plane -transversal with regard to which axis? / 'trasversal' plane = orthogonal to hypothalamic area?) of the "commissura anterior" nerve fibres ??...
At least- IMHO- not the chiasma opticum...because that would be located at the basis of your section, flanked by some bigger blood vessels.....
Hopefully you'll get back to answer more in detail?
For the sake of completeness (and students' convenience) I would like to add the following:
You might consult also specific „Brain atlases“, established well (2D, 3D formats) in / for anatomical studies, e.g.:
- https://atlas.brain-map.org/ , or
- https://mouse.brain-map.org/static/atlas, respectively, and further on:
- https://www.hms.harvard.edu/research/brain/atlas.html , especially:
==> https://www.hms.harvard.edu/research/brain/atlas.html# ; find ‚Distance‘ (below COMP set >- 205 -< & -> 215 Labels and find definition for abbreviations like ACa, etc…[you also can change to
or HIGH RESOLUTION MOUSE BRAIN ATLAS 2D and / or 3D at: https://www.hms.harvard.edu/research/brain/3D_atlas_vDemo.html , select: ->001Anterior commissure (commissura anterior-pars anterior), Anterior commissure (commissura anterior, pars posterior), and Anterior commissure (commissura anterior, pars dorsalis),
Note added: you might consult – besides Handbooks of Anatomy / section: Histology …. several sources –just search for them (brain atlas mouse ; brain atlas rat ; etc. by Googleing at least these key search phrases: just a collection of URL's (neither exhaustively nor complete results):
- https://community.brain-map.org/t/faq-how-can-i-get-a-printed-or-pdf-version-of-the-mouse-human-brain-reference-atlas/411
- http://labs.gaidi.ca/mouse-brain-atlas/
- https://www.frontiersin.org/articles/10.3389/fninf.2018.00093/full
- https://advances.sciencemag.org/content/6/26/eabb3446
- https://www.nature.com/articles/s41598-021-85807-0
- https://www.researchgate.net/publication/329245114_A_Cell_Atlas_for_the_Mouse_Brain
SUNKIN et al, 2012: The Allen Brain Atlas: an integrated spatio-temporal portal for exploring the central nervous system: [https://watermark.silverchair.com/gks1042.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAsAwggK8BgkqhkiG9w0BBwagggKtMIICqQIBADCCAqIGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMlQfPAW7MoJBx2Tb4AgEQgIICc5G1g6abXZYKKWusdg1lqP7KT7PBygOHbN7In8SOOsSkkX_ZIL3q6c98Ugom4uUWFfpD8z-Nb9iyA6YSqT6m55x1uk7akWa5im_e1f0OwxkxdQi8LTORBxrIwk2TI08B7TYCKzzPbGmgBXbtTImpa2BAQAchmn_-lILGJmIr-TsGOwHsRi7Lwt0fqnR3I_mewjTOEVYfHdOvmrWsdLhJl3RG7UpxZbYkACUhoQGohsIhph-XUxjDcXBCYbkCEOM8rllooW4mnswMJbC_VT829knaVpPOMYmccVfyk0SA-YmlXR8Jwj995DIv1GjpbYrWT94WYGgWUwAz-N6JTXODzT3aSEwr176KTdui7tHBpjy_svg2Uv5O-SQkM-4dR_PBRmwjqF8qpQ9_HniIcxmxaTyza-Bdy0TxNAjWR3lhocVuRAFnCprEXom1e1Bqhpzffe0Sn5hSAgHQB2bltPxIxzzUE_AuCtdbD4uaDfkdGRY2elzKTemz8YWFJmJ_ZSpA7KOiK8PA9DYEnzmhCJwr-glbFK2UdfsgMzChqv6UmEUS1NJsafNOlFZ70XpruPHq4oAzwEOzkCzvHcz9MCNa2ZO4CI8_VgKwfEx2xGtqd7hBhi6YTKC0FvbMPe3SYVMnr34oXepQk1eAAgkknRspQIgvBwn9blBORjYzgWKoC9yWjvYlsiYZlmmjnXA8aBeamBe7RCq2e2NkmrhGjtxSzV_VBh_FeKcwmJPxzt3bVG61PE95rUwpkoc6oizka0Cy3h6R_KNQ1FbPRFE-JbRg0KEcGLZ0OtaaK2U-tXysoSLv_-CWUVypcBukB8UWdruGvHHZiA]
- Neuroscience- The mouse reference brain in 3d; Wang, Q. et al. Cell 181, 936–953 (2020), pointing to the ALLEN Brain Atlas once more: https://www.nature.com/articles/s41592-020-0899-4.pdf
- https://jscholarship.library.jhu.edu/bitstream/handle/1774.2/60068/PATEL-DISSERTATION-2018.pdf?sequence=1
- https://www.semanticscholar.org/paper/Paxinos-and-Franklin%27s-the-Mouse-Brain-in-Paxinos-Franklin/0dcc87754c046e8bf9a9081db945f01dca59df0a
- https://link.springer.com/article/10.1007/s12021-020-09490-8, &, &, &…
Good luck and gorgeous results, WHM
Wolfgang H. Muss
thank you for your answer.
it is a transverse sectioning, your guess is correct except that the brain is embedded in Paraffin and I started cutting from the very top and now I can't really know at which part I am exactly. I am trying to know from the structure.
the links are invaluable.
Thank you for your help!
Alaa Skeyni , you're welcome. Sorry for my being back to thread late....
My guess is that it is a tumor. I suggest sharing the slide with a veterinary pathologist.
Dear Alaa Skeyni , hi....would you mind to tell the interested 'Following faculty' what the outcome of your research [i.e. Brain structure or tumor?) finally was?
(Have to admit that I certainly have some interest in, since I did serial sectioning of resin embedded RAT Brain and (virtual by drawings) 3D-Reconstruction of positional relationship of magnocellular nuclear regions surrounding the 3rd ventricle: CA=Commissura anterior, ChO= Chiasma opticum, .... NSO (Nucleus= n. supraopticus) - NPV (n. paraventricularis), NSch (n. suprachiasmaticus), etc. 'back to' the VMN (n. ventromedialis)... for my thesis in Dec.1980, which I 'remember' with thanks and humbleness:
>Die postnatale Ontogenese von.. Die postnatale Ontogenese von..
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