Spectroscopy is used in physical and analytical chemistry because atoms and molecules have unique spectra. As a result, these spectra can be used to detect, identify and quantify information about the atoms and molecules. Application is made of this tool in biosurfactant study when trying to characterize a biosurfactant molecule. Fourier transform infrared spectroscopy demystifies the functional groups in biosurfactant molecules in preparation for mass spectroscopy which is frequently employed for structural characterization. The Gas chromatography - Mass spectrometry technique combines the separation properties of gas-liquid chromatography with the detection feature of mass spectrometry to identify different substances within a test sample based on characteristic fragmentation patterns at specific retention times. Literature is replete with these information. Just take some time and go through them.

Thank you Maurice for this quick answer and for the pdf file

may be a biosurfactants, but to confirm you should optimize the experimental condition (in batch cultures for 96 hours at optimum conditions), for measuring Surface tension measurement ( tensiometer) , Emulsification index (E24), Drop collapsing test or others qualitatives tests.