I already checked four media, PDA, SDA, CDA and mycoocy. but get nothing only mycelium grows well.
Thanks in advance
Hi, try to grow it on carnation leaf on water agar. You will get spore from the sporodochia.
This depending on incubation period, increasing incubation period more than 72h Increase your chances of getting spores... good luck
As the coleague Oadi Matny said, you must try to grow it on carnation leaf on water agar.
or...
using Spezieller Nahrstoffer agar (SNA) (see Leslie and Summerell 2006), this last one (SNA) is the recomended medium for Fusarium sporulation.
I recommend using dianthus caryophyllu leaves,
immersed in water agar, sow the mycelium Observe the growth for a week, until the nutrients are exhausted and start sporulation
Hallo, i recommended using a poor media like agar agar and keep the culture under alternative light 12h light and 12 h. dak at temperature 25c to push the mycelium to sporulate. good luck.
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