I am planning for an experiment using MitoTracker. I wish to do time lapse study in vitro. I want to know about the stability of the mitotracker dyes. For how long is the fluorescent intensity maintained? Is it possible to add the dye, then perform the experiment and then visualize the cells. The experiment will last approximately 48 hours. If not, can you suggest some alternative for the same?
I used MTT and crystals formed after 4 hrs.I did not dissolve in DMSO and observe the adherent cells for 48 hrs. the crystalline structures are stable. I am not aware of an y other dyes
I don t know for mitoTracker, but if the dye is not stable enough for a 48h video, you can try to transfect/infect your cells with mtDsRed plasmid/virus. the fluorescence might bleach at some point so you should try to acquire at a low frequency. For that, you need to be sure that this frequency is appropriate for analysing the biological process (velocity, morphology, fusion/fission) you want to study.
We used MitoTracker Red in HepG2 cells but the cells were fixed. From my experience with similar dyes I would say that it will be difficult to have a good signal until 48h.
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