Hello everyone.i want to ask what is The sequences of forward and reverse primers for the abscisic acid gene?

More Ammar Syazwan's questions See All

Thermophysical properties of MWCNTs nanoparticles?

Is there any article can I find all thermophysical properties of MWCNTs nanoparticles especially, thermal expansion coefficient and electrical conductivity?

30 June 2024 4,075 2 View

Exergetic effeciency analysis?

How to calculate the the exergetic effeciency of heated pipe with a constant heat flux?

30 June 2024 1,039 1 View

Please can you help me identify this specie of Euphorbiaceas family?

I found this ornamental plant in the garden please help me identify this specie.

30 June 2024 4,499 8 View

Are there recent publications on the economic comparison of Solar PV Curtailment vs. BESS Installation?

I'm currently exploring the economic implications of solar PV curtailment versus the installation of battery energy storage systems (BESS). Specifically, I'm interested in recent publications or...

27 June 2024 6,423 2 View

Fluid flow through copper tube?

What does it mean meshed wall and shell conduction with or without run-out method use to meshing copper tube in Ansys fluent?

07 June 2024 9,030 3 View

Can I find a fast journal (indexed in scopus and without fees)?

Good greeting. I want to publish a paper review in a journal specialized in food, health, toxicology, or even environmental sciences, provided that it is indexed in Scopus. I hope you can help...

19 May 2024 4,426 4 View

Can I perform Duncan's test using Multivariate ANOVA?

Considering having a data of plants that consists of several types of plants with several treatments and for each treatment four replicates. Moreover, I have multiple continuous outcomes for each...

01 May 2024 2,916 0 View

What materials or configurations hold the greatest potential for enhancing the efficiency and stability of double perovskite solar cells?

I'm currently exploring double perovskite solar cells and seeking advice on the most suitable materials or configurations for further investigation. My focus is on optimizing solar cell efficiency...

26 April 2024 1,745 0 View

Which are the suitable under relaxation factor to ensure a better convergence in the laminar mixed convection in Ansys Fluent?

which under relaxation factors is suitable for laminar mixed convection when using SIMPLE algorithm for velocity-pressure couple, PRESTO! and second order UPWIND schemes for pressure, momentum and...

19 April 2024 6,162 0 View

I found the local temperature difference in circular tube under laminar mixed convection condition using water great than 10 dgree, is it correct?

I need to explain the local temperature differnce and boussinsq hypothesis validity?

05 April 2024 6,136 7 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

Paul Rutland

I am sure it would depend on the species of plant you are working with and if it is the genomic sequence or cDNA that you are amplifying

Ammar Syazwan

The species that i use is Neolamarckia cadamba.

Rik Verdonck

Are you sure there is such a gene? Abscisic acid is not a protein...

Arnaud Cheuk

ABA biosynthesis consists of many reactions. So I'm not sure there's a gene that could directly encode for ABA