This is for PCR amplification.
I am sure it would depend on the species of plant you are working with and if it is the genomic sequence or cDNA that you are amplifying
The species that i use is Neolamarckia cadamba.
Are you sure there is such a gene? Abscisic acid is not a protein...
ABA biosynthesis consists of many reactions. So I'm not sure there's a gene that could directly encode for ABA
Hi, I am working on 48V Lithium-Ion battery pack. I am wondering if there is fast MOSFET (or any other technology), to control the current of charging and discharging the battery with high...
20 January 2021 1,717 4 View
Hi! i am running a simulation using a cluster and I have encounter an error during my nvt equilibration like this: Cannot rename checkpoint file; maybe you are out of disk space? I deleted some...
03 January 2021 3,544 2 View
Modification of the rheological properties of asphalt
25 December 2020 7,390 2 View
I am trying to work on reducing the PAPR of the FBMC/OQAM with the TR and PTS methods. for that if someone can share with me one of these matlab codes. my e-mail: [email protected]
19 December 2020 7,764 1 View
How to estimate the acidity of a cheese?
13 December 2020 4,890 4 View
I am going to clone gDNA fragment > 10 kb into a pCR TOPO vector. I performed the PCR using KOD PLUS Neo polymerase for 3-steps PCR reaction, I am not able to find the specific size. Several an...
03 December 2020 1,939 5 View
does anybody knows how can i get the change in the distance between the notch nodes (two points showing in the figure ) vs the applied force in every step as a table out put or curve in...
23 November 2020 352 3 View
Hi I got a low confidence index in the EBSED analysis even after days of polishing using 1 to 0.02 um suspension using 1 lb force, the sample was Al/W composite (has intermetsllic interface)...
26 August 2020 3,547 4 View
hi please I have Al/W sample and I am looking for screw dislocations on the intermetallic surface, my question is: Should the sample be etched? and should I use the SEM or TEM microscopy? what...
06 August 2020 9,703 2 View
I am using google colab to train a model for sign language recognition using this dataset: https://www.kaggle.com/feronial/turkish-sign-languagefinger-spelling I tried different architecture...
08 May 2020 4,098 5 View
I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...
03 March 2021 8,066 4 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...
02 March 2021 4,443 2 View
02 March 2021 3,060 3 View
how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...
02 March 2021 811 3 View
What do you know about biotechnology art?
02 March 2021 1,653 3 View
I am searching for a good place for the Post Doc,
02 March 2021 4,053 3 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...
02 March 2021 8,437 2 View
Please provide Book Title and author name
02 March 2021 9,059 3 View