Store in -80c. RNA is pretty stable for long times, even prior to extraction, at cold temp. Commercial products such as RNA Later can be used (or made using a home-brew recipe), but are largely unnecessary for most applications.
Hi Hamdia,
in my experience freezing blood samples without preservatives at -80°C is not enough. At the latest when thawing your samples for further processing damaged cells will have released sufficient amounts of very stable RNAse that degrade RNA. But even in the freezer RNAse in your sample will do its slow but detrimental job. I would recommend specific sample collection tubes such as PAXgene RNA or others. Or search for a self-made much cheaper solutions.
Best regards
Collect the blood into a paxgene tube (http://www.bdbiosciences.com/us/applications/blood-collection/cell-biomarker-preservation/paxgenereg-blood-rna-tube/p/762165). These are specifically designed to stabilize RNA for extraction from whole blood.
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