Does anyone have or know about a protocol for isolating microvessels from mouse brain to study tight junction proteins?

There are different protocols for microvessel isolation from mice or rat. Which protocol should I follow to isolate microvessels from mouse brain so that I can evaluate different tight junction...

05 January 2021 696 2 View

How can I use an hybridization of ontology and machine learning for text summarization ?

my work consists of creating a system for text summarization in a specific domain(crisis management) using a hybridization of ontology and machine learning algorithme ,my question is how to use...

23 February 2020 8,327 2 View

Gauge Pressure and Operating pressure on combustion system?

Hi all, Please I have a question about the pressure: I am working on combustion, and I want to study the effect of pressure in my geometry, I couldn't understand where sould I put the pressure for...

14 February 2020 7,977 3 View

What will happen if I add the NO models (NO thermal and NO prompt) for the flamelet model using GRI mech? How Fluent works in this case?

Hi everyone, The GRI mechanism contain all the reactions, and species of NOx, and in the Fluent we can also find the NO models ( prompt, thermal and N2O..), when I added the models I found a big...

22 December 2019 3,785 5 View

Is there a difference in binding site when comparing anti-CD279 (PD-1) clones EH12.2H7 and EH12.1?

Hi all, I would like to know if these clones of anti-PD-1 antibodies (EH12.2H7 and EH12.1) share the exact binding site. Thank you in advance for your help, Sabrina

19 March 2019 4,908 3 View

Is the title and goal of my project suitable?

I am starting works on a project with current working title: Gender Roles in African Philosophy: Appraising the place of women in African traditional society. A Case Study on Setswana and...

20 January 2019 2,161 4 View

How to fix the Error: The mesh file exporter could not resolve cyclic dependencies in overlapping contact regions?

How to fix the Error: The mesh file exporter could not resolve cyclic dependencies in overlapping contact regions. Please try to Repair Overlapping Contact Regions.

01 December 2018 7,320 3 View

What to use for correlation between ordinal scales?

Hello, I have a variable "firm's results" which is an ordinal scale and 5 variables for direct marketing, each of them in ordinal scale too. I know that I can use Pearson if I want to know the...

05 November 2018 1,535 3 View

I have tilt in my geometry, so Is there any way for doing Line moving with the flow ?

Dear professors and research scholars, I have a query for the code Fluent My geometry is not symmetric, I have tilt in my geometry so Is there any way for doing Line moving with the flow !!!...

19 February 2018 8,538 4 View

I have a problem with my results from FLUENT when I imported in ORIGIN, I have other lines! Does that mean the problem with the mesh or secondary air?

Dear professors and research scholars, Attached is a figure showing the distribution of the temperature along the central axis of combustor Fuel, I have problems with my results, When I put it in...

09 February 2018 738 2 View

Which is the most efficient etching solution for nickel based alloys?

Which solution reveals the microstructure and features of nickel-based alloys?

02 March 2021 2,221 3 View

How can I selectively dry etch Ti on a polymer substrate?

24 February 2021 5,378 1 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View

Is it possible to highly selective remove a fused silica template from PDMS by laser-induced backside wet etching (LIBWE) method?

Hi everyone I'm trying to pattern circular cross-section microfluidic channels in PDMS. The template is an optical fiber with an axially non-uniform diameter. To the best of my knowledge, there...

22 January 2021 2,352 2 View

What is suitable etching resist coating for polished Titanium Grade 5 surface?

Currently we start a chemical etching project. This project involved etching a polished titanium grade 5 surface part. We facing a problem where by our etch resist coating peel off during etching...

15 January 2021 1,592 3 View

How will DeepMind AlphaFold2 solution of the core problem of protein structure prediction from sequence impact biology and biophysics?

AF2 may most strongly impact experimental determination of protein structure by multiple methods with possible benefits and negative impacts over time. Some areas may need to quickly pivot or...

13 December 2020 6,026 5 View

How to Strip off HSQ( Fox 16) after etching?

We are finding a hard time to strip of residual HSQ ( Fox 16) after using it as a mask in etching? We are coating 600 nm thick HSQ and writing with 3000 microC/cm^2 dose at 1 nA current. We are...

08 December 2020 5,452 2 View

How to realize silicon etch using PDMS stamp?

I am trying to make silicon etch with KOH, using a silicone stamp similar to PDMS. So I did the manual bond between silicone stamp and silicon but when I put them in KOH solution they separate...

18 November 2020 7,114 3 View

What would be the optimum gel concentration to resolve dsDNA fragments from 10 to 16 base pairs in size?

Hey scientists, I am intending to observe the extension of a short 10-mer primer on a 16-mer template. I just use a certain DNA polymerase to extend the primer on a templating strand with a...

04 November 2020 6,666 8 View