Hello everyone! I need to label mouse PV inhibitory neurons in the medial prefrontal cortex at an early post-natal time point (P7). However, I am experiencing some problems finding a good antibody to achieve this purpose, since these neurons still do not express PV at this time point (they only start expressing around P14) and I require a strong and, preferably, a nuclear labeling. Can you point me to good antibody for immunohistochemistry in frozen slices?
Thank you in advance for your help.
Igor O. Nasonkin
try ab11427, rabbit polyclonal, for example see my older paper Article Long-Term, Stable Differentiation Of Human Embryonic Stem Ce...
(tissue was fixed with PFA, not methanol). Very strong crisp staining. Good luck
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