In the experiment, the protein concentration is fixed why the drug concentration varies
I want to download the amber99sb-ildn force field in gromacs to study a protein-ligand interaction using a serum protein and a TCM as a ligand, but the field cannot be seen on the gromacs tutorial...
29 November 2023 3,048 1 View
I want to visualize the common genes generated from the string database. When sent directly from the String database for visualization my genes are clustered so tightly that I can not tell which...
15 January 2023 4,002 1 View
I generated the complex of my protein and ligand and visualise it in Chimera and other visualisation software but I observed no interaction between my protein and ligand. What could be the cause...
21 February 2022 5,484 3 View
Why is my protein topol.top file like this? attached is the file
19 February 2022 6,869 4 View
how can I fix this error in gromacs, ERROR 37 [file ligand.prm, line 41]: Incorrect number of atomtypes for dihedral (0 instead of 2 or 4)?
15 February 2022 4,142 2 View
I have the ligand perimeter in my working folder and file but I am receiving an error in gromacs that says "Fatal error: Topology include file "ligand.prm" not found", how can I fix this, please....
14 February 2022 9,952 3 View
How can to fix this? In command-line option -f Invalid command-line options File 'ions.mdp' does not exist or is not accessible. The file could not be opened. Reason: No such file or...
12 February 2022 2,518 3 View
How can I use ignh to ignore water molecules in a gromacs molecular dynamic simulation?
11 February 2022 7,801 1 View
I doing MD simulation of protein-ligand interaction at two different temperatures 273K & 310K, how can I do this?
08 February 2022 686 4 View
How can I create user-defined function using stern-volmer equation(Fo/F=1+Ksv[Q]) in originlab software. what are the parameters needed to build the stern-volmer user-defined fitting?
09 November 2021 5,292 0 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
For a grid connected BESS (lets say 100 MW/200 MWh system, LFP battery), if we charge the BESS in such a way that whenever we can access power from the solar that will charge the BESS. Hence it...
01 August 2024 9,903 7 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...
24 July 2024 8,696 0 View
how to fix this error, from phq_readin : error # 1 DFPT with the Blochl correction is not implemented I am attaching my ph.in file.
23 July 2024 4,649 2 View
Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...
19 July 2024 2,155 0 View
> library(meta) Error: package or namespace load failed for ‘meta’ in loadNamespace(i, c(lib.loc, .libPaths()), versionCheck = vI[[i]]): there is no package called ‘numDeriv’
18 July 2024 3,536 0 View
I have a problem with geo-referencing a shape file. How can I fix it when it appears in mines despite selecting WGSWGS 84 / UTM zone 38N?
18 July 2024 2,696 1 View