Sher

Even though most of the references mentioned the use of potassium acetate the ref. below showed the use of sodium acetate

---https://books.google.com.sa/books?id=JdeZDwAAQBAJ&pg=PA54&lpg=PA54&dq=sodium+acetate+and+aluminum+chloride+in+total+flavonoid&source=bl&ots=hTsPNfZRT9&sig=ACfU3U2jG5SqSytFhq_MlCcH8LXNoTqySg&hl=en&sa=X&ved=2ahUKEwi49LbU5MLqAhUIFpoKHRGxBQ4Q6AEwC3oECAoQAQ#v=onepage&q=sodium%20acetate%20and%20aluminum%20chloride%20in%20total%20flavonoid&f=false

Good luck

Dear all, similar question discussed the same subject at RG. Yes sodium acetate is used, see the first attached file, the second one arises the importance of the solvent. My Regards

https://www.researchgate.net/post/Please_help_about_the_total_flavonoid_content

https://www.researchgate.net/post/Does_Total_Flavonoid_Content_determination_requires_Potassium_Acetate_as_reagents_when_Quercetin_is_used_as_standard_compound

I agree with Adel Amer

Hi;

Yes, you can replace potassium acetate with sodium acetate. Both are a good source of acetate ions. The attached document mentions the use of sodium acetate.

With my best regards

Dear Jan Sher

I agree with Abdelkader BOUAZIZ . You can use sodium acetate instead of potassium acetate in that test.

Regards

Dear Jan Sher I just found an article entitled "PROBLEMS WITH ULTRAVIOLET SPECTROSCOPY OF FLAVONOIDS USING SODIUM ACETATE IN METHANOL". It's fairly old (1985), but it might be worth reading.

Thank you everyone

of course, the main purpose of the presence those compound in flavonoid test is to provide an acetate ion

Dear Muslem Muslem I agree with you in that the acetate ion is what counts. As a chemists, I think that sodium or potassium should make no difference. Jan Sher: On the other hand, both chemicals are rather cheap. So, when the literature procedures asks for potassium acetate, why not using it?

Dear Prof. Frank T. Edelmann, I think there is a slight difference regarding the dissociation constants and the size of the cations counterions (K+, Na+). My Regards

Abdelkader BOUAZIZ That's certainly true.

Frank T. Edelmann , Yes i am agree with you, but recently we have Sodium acetate in our laboratory not Potassium acetate.

Yes you can replace it. I used sodium acetate for flavonoid test.

Dear Jan Sher if the use of potassium really counts, you could easily prepare potassium acetate by yourself e.g. by neutralizing acetic acid with potassium hydroxide or by the reaction of acetic acid with potassium carbonate. In both cases you just need to evaporate the resulting solutions of potassium acetate to dryness.

Yes you can. You can as well use rutin as a standard if both acetate is not available.

yes you can add