I am looking into remove organic compounds from water by adsorption using porous materials. My question is on how to calculate the required amount of adsorbent and selecting of concentration of adsorbate? And also, after adsorption, the adsorbed amount will be measured by using HPLC. When the calibration is drawn, the standards should be also treated with conditions used  such as temperature and duration of shaker in adsorption? 

Thank you so much.

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