we often use ultra waves at 20 kHz to break cells, perhaps could you try with your adipocytes.

I want to say ultra sounds !

Thank you so much for your answer. Can you please suggest should I directly apply lysis buffer or should I trypsinize follwed by lysis buffer?

I guess you don't need to trypsinize, add directly lysis buffer and apply ultra sound with the probe diving in the medium. Keep your culture flask in ice to avoid overheating.

Thank you