Hello anyone, can you please help to get rid of these negative peaks? and what i wish to find out the methanol in NaHCO3 using this column?
Thank you in advance.
Maybe your column is contaminated. Wash it well. By the way, what are your sample and mobile phase? Are they both polar?
Your question does not make any sense as posted. Could you include complete HPLC chromatograms (with scales and parameters) to illustrate what you are inquiring about? Also, your complete HPLC method details too. With this basic information, it may be possible to offer advice.
Thank you, everyone, for your comments. We have successfully resolved the issue by setting up the polarization curve(polarity matches of sample with elluent) and getting good results. Thank you every one
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