As BanKit is not accepting the nucleotide sequence and say'' Warning: The protein translation of your sequence contains internal stop codons. CDSs with internal stops are not biologically correct and cannot be accepted if they are submitted; they will be returned to you for correction before accession number is assigned. Please correct the CDS intervals (and reading frame if the CDS is 5' partial) to add your CDS features. ''
Of course. Do six frame translation with nucleotide output and choose the best/longest frame.
Yes.. thank you. I want to do it but is it authenticated? There are so many sequences in NCBI which also contain internal stop codons. How they were sequenced without trimming?
Lakee Sharma If you find something which should not be there on NCBI you can contact them and ask for explanation/correction. But for your own data, submission of CDS requires to be without internal stop codons.
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