Lakee Sharma

10 Questions 10 Answers 0 Followers

Questions related from Lakee Sharma

How to divide homologs in different groups and subgroups in a phylogenetic tree?

I'm working on the phylogenetic assessment of transcription factors. In every literature, scientists have categorized the homologs into groups (like I, IA, II, III, IV, etc). I want to know that...

14 February 2022 9,892 0 View

What is the way to assign a number to the transcription factor?

Hello all, I have been working on in-silico analysis of plant TFs. I found that there is a numbering after each TF like OsWRKY1, TaDREB15, etc. I am not able to find out how to assign these...

31 January 2022 2,736 4 View

How to publish a paper with a new gene (Gene is not submitted in NCBI)?

Hello everyone.. I am working on the role of transcription factors in abiotic stress tolerance in plants. For this, we have found a new gene (which is not available on NCBI/Genbank) from the...

08 June 2021 9,886 5 View

What is the role of DPPH and H2O2 in abiotic stress tolerance?

Hello everyone, I'm doing some experiments with plants by exposing them to abiotic stress. I have estimated the DPPH inhibition percentage but don't know about what should be the trend (either...

15 February 2021 7,494 2 View

How to use plant root tissue for experimental analysis?

Hello everyone, I am working basically on plants (hydroponic culture). I want to estimate biochemicals and gene expression pattern of root tissues. My question is that how to harvest the tissues...

21 January 2021 4,651 3 View

How to generate hairy roots without Agrobacterium under in- vitro condition?

I want to establish hairy roots of my plant for abiotic stress treatment, but I want to do it without involvement of Agrobacterium. In every previously done research works, researchers have used...

23 October 2020 6,654 7 View

How to get rid of green contamination during hydroponic culture in Hoagland?

Hello everyone, I routinely used to make Hoagland solution for the culture of seedlings. After 5-7 days green contamination appears on the bottom of the flask. However I always autoclave the...

16 March 2020 4,885 4 View

Which result should I follow when the alignment result of the same sequence is different in the case of BLASTn and BLASTp?

I am working on WD protein, which nucleotide sequence was obtained after sequencing. When I proceeded for sequence alighnment to check the most similar homologs, I have found that result of...

02 March 2020 8,355 8 View

Can i proceed with cDNA having 260/280 value around 1.4 and 260/230 values around 1.3?

I used to synthesize cDNA from the RNA, extracted from the leaves by TRIZOL method. Im not getting a good 260/280 as well as 260/230 values. Should i proceed for further experiments?

23 February 2020 3,248 4 View

Hello, can anybody explain the proper procedure for the removal of stop codons from the cDNA during submission in BanKit??

As BanKit is not accepting the nucleotide sequence and say'' Warning: The protein translation of your sequence contains internal stop codons. CDSs with internal stops are not biologically correct...

19 November 2018 825 3 View