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Questions related from Lakee Sharma
I'm working on the phylogenetic assessment of transcription factors. In every literature, scientists have categorized the homologs into groups (like I, IA, II, III, IV, etc). I want to know that...
14 February 2022 9,849 0 View
Hello all, I have been working on in-silico analysis of plant TFs. I found that there is a numbering after each TF like OsWRKY1, TaDREB15, etc. I am not able to find out how to assign these...
31 January 2022 2,661 4 View
Hello everyone.. I am working on the role of transcription factors in abiotic stress tolerance in plants. For this, we have found a new gene (which is not available on NCBI/Genbank) from the...
08 June 2021 9,837 5 View
Hello everyone, I'm doing some experiments with plants by exposing them to abiotic stress. I have estimated the DPPH inhibition percentage but don't know about what should be the trend (either...
15 February 2021 7,431 2 View
Hello everyone, I am working basically on plants (hydroponic culture). I want to estimate biochemicals and gene expression pattern of root tissues. My question is that how to harvest the tissues...
21 January 2021 4,562 3 View
I want to establish hairy roots of my plant for abiotic stress treatment, but I want to do it without involvement of Agrobacterium. In every previously done research works, researchers have used...
23 October 2020 6,613 7 View
I am working on WD protein, which nucleotide sequence was obtained after sequencing. When I proceeded for sequence alighnment to check the most similar homologs, I have found that result of...
02 March 2020 8,307 8 View
I used to synthesize cDNA from the RNA, extracted from the leaves by TRIZOL method. Im not getting a good 260/280 as well as 260/230 values. Should i proceed for further experiments?
23 February 2020 3,212 4 View
As BanKit is not accepting the nucleotide sequence and say'' Warning: The protein translation of your sequence contains internal stop codons. CDSs with internal stops are not biologically correct...
19 November 2018 782 3 View