Does anyone know, can i do cryosectioning of whole aorta after oil red O staining and use the cryosection parts for further analysis?

I would like to quantify the lesions on aorta. I want to take image of atherosclerotic plaques formation in aorta by using O red Oil Solution. After the staining, what will be the storage...

16 February 2021 5,876 2 View

What is the best solution for counter electrode for perovskite solar cells at room conditions?

I'm investigating transparent counter electrode for FTO/PEDOT:PSS/MAPI3/PCBM layered perovskite solar cell. In my research lab, currently i have a glove box with N2 gas as a carrier. I'm goning...

21 December 2020 6,504 3 View

My status has changed from "Awaiting Reviewer Score" to Awaiting AE Decision". Could the Associate Editor reject the manuscript at this stage?

Otherwise what this status means. If the Editor has received the comments from reviewer then why he is not sending it to Authors. As 10 days already spent till the status changed.??

18 November 2020 6,252 4 View

How to adapt a mature Questionnaire?

I am looking forward to seek some help regarding adaptation of mature questionnaires. I am new to quantitative studies and wants to use some demographic in my questionnaire, is it necessary to add...

08 November 2020 7,146 5 View

Is there a way to isolate nuclei without purifying cytoplasmic protein aggregates?

Hello, I am trying to isolate nuclei from HEK293T cells expressing a protein that aggregates in the cytoplasm, however when I run my samples in western blot the cytoplasmic protein is strongly...

02 November 2020 9,541 3 View

How can i fill zinc oxide or titanium oxide into etched pattern on glass substrate?

Hi! I need zinc oxide or titanium oxide to be filled into etched area on glass sbstrate. Etched are is being photolithography processed and being dry etched. Material must not present around the...

25 October 2020 152 7 View

How to get the most out of your qPCR data?

23 October 2020 7,537 3 View

Do you think that the e-learning that took place after the COVID 19 crisis was fair for students to evaluate their knowledge?

The significance of the question is to know the extent of accuracy in evaluating students.

19 September 2020 3,663 13 View

How to calculate reliability for a newly launched system?

We have a hydraulic system (water collector and distributor) which is newly started, how can we calculate its reliability?

07 September 2020 6,619 2 View

Neonatal Renal dose adjustment! Guidelines or the reference that is most authentic in clinical practice.??

In the infants to the adult age group, we have been given a concrete guideline for dose adjustment on the basis of CKD-Stages or creatinine clearance. this pathway is missing in neonates but...

07 September 2020 1,731 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

How to find the mean, SD and CI in SPSS?

Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....

03 March 2021 506 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Which reagent is best among 'Dimethylglyoxime" and "Dithiothreitol" for spectroscopic determination of Nickel ?

What I think is Dithiothreitol. As it is water-soluble and does not form a precipitate. But, the thing is it contains sulphur which is quite hazardous to aquatic animals.

01 March 2021 8,374 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View