Heat the sample during nitrogen evaporation of peptides eluted in ethanol/6 N hydrochloric acid (1,000:1, v/v)?

03 March 2014 1 9K Report

I am doing nitrogen evaporation of a sample of proteins eluted from a sep-pak carteridge in ethanol/6 N hydrochloric acid (1,000:1, v/v). The protein of interest is Oxytocin.

My question is: should I use heat the sample while evaporating? I would think that heating would cause more rapid evaporation, but cold temperature would be safer for protein integrity.. However, will the EtOH/HCL solvent evaporate without heat??

Any thoughts and comments are greatly appreciated.

Tushar Kanti Ray

If your target material is stable in that kind of high-HCL environment I think you can safely use heat.

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