Nicolai Bonne

2 Questions 9 Answers 0 Followers

Questions related from Nicolai Bonne

How do I perform an ELISA validation?

I am performing a (partial) validation of commercially available ELISA kit. I am using the kit on a previously untested matrix, so I wish to validate the assays Accuracy, intra-assay Precision and...

04 April 2014 8,362 4 View

Heat the sample during nitrogen evaporation of peptides eluted in ethanol/6 N hydrochloric acid (1,000:1, v/v)?

I am doing nitrogen evaporation of a sample of proteins eluted from a sep-pak carteridge in ethanol/6 N hydrochloric acid (1,000:1, v/v). The protein of interest is Oxytocin. My question is:...

03 March 2014 9,173 1 View