Hi
I would like to ask is it possible to do heat inactivation of serum samples without water bath. We are dealing with over 200 human serum samples and our water bath capacity is limited. Looking forward to hearing suggestions and personal experiences
Regards
Zohaib if water bath size is problem then go for manual heating serum samples can be deactivated up to 55-58 for 30 min so I think use heat plate and a pot with water. First adjust the water temperature for 5-10 min on heat plate. when you are confirmed that it can be maintained then go for next step. Good luck
Depends on what type of viruses are there, but I will suggest incineration of the serum, if a nearby hospital with incineration facility is available.
Zohaib if water bath size is problem, I suggest to use samples according to your water bath size, Do you have to in activate your serum samples all at the same time?
The limited capacity of your water bath is not an obstacle. I think, that every half hour you will remove the treated tubes and deliver new ones and so on. The inactivated sera will be stored in freezers at - 20 ° C untill analysis. Greetings
I suggest you use an incubator and set it to 40 degree Celsius for about thirty minutes or more.
Zohaib, use deep-well tube plate that sizes equal to ELISA plate, and would more adjustable in your lilliputian water bath.
I also had to heat-inactivate a large number of samples for neutralization assays. I put them in 96-tube racks in a spare incubator set at 56C. I put the plastic racks in first to pre-heat them and added another 5 minutes to the incubation time to let the tubes equilibriate.
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