When extracting lipids with the MTBE method, the indicated temperature is RT. In addition, drying the samples in a speedvac is mandatory due to the presence of water and such procedure is long and heats them up.
Do any of you get degraded or oxidized lipids? Do you use an antioxidant regularly? which one, BHT or DTT?
Also, I use glass for the B&D protocol due to the presence of chloroform, but I see that microcentrifuge tubes are commonly used for MTBE. Do you detect plastic residues in your MS spectra?
Thank you.
Hi Rita,
I often use MTBE solvent for lipid extraction. I generally use BHT (50ug/mL).
Concerning the eppendorf tubes, my suggestion is you use PTFE eppendorfs tubes they are (according to the manufacturers) resistant to plastic bleaching. They are a bit more expensive than regular ones. I haven´t had any contamination issues with the MTBE extraction method.
Hope this helps,
Ana
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