When you have to deal with a very large number of samples, it is impossible to buy the reagents. I feel , it is possible to prepare reagents locally and reduce the cost.
Is it possible to stop river water going waste to sea? Would there be any harm to us, to the sea animals, plants near the river-sea junction if fresh water is not allowed to reach the sea? would...
05 February 2019 7,981 3 View
Any suggestion of nano powders made of natural fibers that can be reinforced with natural fiber polymer matrix composites?
23 November 2015 8,265 8 View
In water demineralization, when I have the following anions in mixture: chloride, fluoride, nitrate, nitrite, arsenate, sulfate, phosphate, when the resin is getting saturated, what is the...
06 November 2014 1,783 8 View
n-3, n-6 fatty acids are important nutraceuticals. Natural sources are not always dependable.
16 October 2012 1,607 1 View
Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
25 February 2021 3,443 4 View
I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.
24 February 2021 1,633 4 View
My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...
21 February 2021 5,656 3 View
I try to transfect MDA MB 231 cell line with a plasmid by PEI, unfortunately, the rate of transfection is low, and now I wanna find and order the best transfection reagent for this cell line and...
19 February 2021 828 6 View
I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...
17 February 2021 7,402 4 View
Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...
17 February 2021 5,399 1 View
Dear friends! I am back to work after 3 years career break. Now performing a PCR, feeling like a newbie... Please, help to understand whether RT-PCR reaction "worked" or not. Which results should...
14 February 2021 3,129 8 View
I have been looking for a good cost-effective tool for 3D modelling a glass-glass solar module system. I was initially considering Skelion as a tool but it does not look very promising, a bit...
13 February 2021 8,126 1 View
In order to measure the polyphenols in broccoli, we prepared a reagent for measurement using the following procedure. 1. Extract the crushed plant with 70% methanol. 2. Mix 1 ml of the extract...
07 February 2021 845 4 View
The parameters of the curve all seem acceptable, typically reactions results are above 0.9852, -3.1 to -3.6 slope, and 90-110% reaction efficiency. The results seem to be what I would expect from...
04 February 2021 1,138 3 View