Dear all,
My research group is currently working with a 12-litre fermenter. Somehow, if we do the fermentation for more than 20 hours, the process will be more prone to contamination. Our current suspect is the air vent filter. As we live in a tropical country, the humidity is quite high (40-50% with the HVAC system). The current material of the air vent filter is PTFE. Have you ever experiencing this problem before? Thank you!
Khrisna Putera Buddhiman , is the filer you refer to on a feed line to your fermenter or on the vent of the fermenter? From your question I assume the vent line. In this case run the vent line to a container of sterilized solution and submerge the vent line in the container. If the vent loops over the top of the vessel down to the side, none of the liquid should enter the fermenter. If somehow atmospheric air is drawn through the liquid in the container, is should be sterilized.
Hello, you can chech your vent air by passing trough liquid media in falsck for 1-2 hour and then see any grow after incubation of flask about 48hour.
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